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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in several tumors, which results in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial purpose in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two more splice variants ended up reported but specific nucleotide sequence details isn't still available.

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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide generally incorporates the epitope identified because of the antibody.

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This guide outlines even further possible optimization of mobile and immunoassay parameters to make sure the absolute best success are...

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This manual outlines even more feasible optimization of mobile and immunoassay parameters to make certain the best possible benefits are...

Antibodies certain to the blocking peptide no more bind for the epitope within the goal protein. By comparing the staining from your blocked antibody compared to the antibody by itself, you can see which staining is precise.

Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose users are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which lead towards the temporal coordination of each and every mitotic function. Cyclin E varieties a complex with and features like a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S period boundary and is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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